Авторы: Мирзаева Ю. Т., Усманов П. Б.

Рубрика: Тезисы

Опубликовано в Биоэкономика и экобиополитика №1 (1) декабрь 2015 г.

Дата публикации: 30.01.2016

Статья просмотрена: 1 раз

Библиографическое описание:

Мирзаева Ю. Т., Усманов П. Б. Effect of Diterpenoid Alkaloid 14-O-Benzoiltalatizamin ON NA+/СA2+-Exchanger Smooth Muscle of Cells of an Aorta of a Rat // Биоэкономика и экобиополитика. — 2015. — №1.



 

Na+/Ca2+-exchanger, plays an important role in maintenance of Са2+ -homeostasis in a cage and therefore regulation of its activity has special value and is provided with a number of mechanisms. Found by us ability of alkaloid 14-O-BT to inhibit reductions of smooth muscles in conditions when Na+/Са2+-exchanger works within the turned mode and transfers ions of Са2+ to MMC (the smooth muscle of cages), testifies to prospects of further research of these connections for development of effective approaches of correction of pathological states the overloads arising at Са2+. Suppression by alkaloid 14-O-BT, reductions of the preparations of an aorta of a rat induced by Krebs's solution without Na+ also is the evidence of interaction of these agents with Na+/Са2+-exchanger of SMC.

As our checked experiments, replacement of normal solution of Krebs by solution without Na+ showed, causes immediate reduction of preparations of an aorta which within 10-15 min. reaches the maximum amplitude making 70,1±2,3% of the control reduction induced 5 microns of the adrenaline (taken for 100%). In these experimental conditions, addition of a blocker potential - dependent channels Са2+ of verapamil (1 micron), was followed by reduction of force of reductions of the preparations of an aorta induced without Na+-solutions to 53,4±3,6%. Such reduction of force of reductions of the preparations of an aorta induced without Na by +-solution is explained by suppression verapamil of receipt of ions of Са2+ in SMC through potential - dependent channels Са2+.

Results of these checked  experiments convincingly testify that development of reductions of the preparations of an aorta induced without Na+-solutions in the presence of verapamil generally is provided with ions of Са2+, coming to MMC through Na+/Са2+-exchanger.

As showed our researches, in these experimental conditions alkaloid 14-O-BT considerably suppresses the reductions of preparations of an aorta induced without Na+-solutions. Thus the effect 14-O-BT had dose-dependent character and at its concentration of 10 microns force of reduction of a preparation of an aorta induced without Na+-solution decreased by 20±3%, and at its concentration of 160 microns it as much as possible suppressed reduction force for 63,9±2,8% of the control received in the presence of 1 micron of verapamil and taken for 100%.

Основные термины (генерируются автоматически): dependent channels Са2+, aorta, reductions, preparations, experimental conditions, reduction, verapamil, suppression verapamil, immediate reduction, transfers ions, control reduction, important role, special value, reduction force, microns force, pathological states, smooth muscles, maximum amplitude, smooth muscle, effective approaches.

Обсуждение

Социальные комментарии Cackle

Посетите сайты наших проектов

Задать вопрос